Gano Kai tsaye na Maganin Cutar Kwayar cuta

Waɗannan jerin hanyoyin hanyoyin tantancewa ne ta hanyar amfani da takamaiman antigen na hoto don gano ƙwayoyin rigakafi a cikin maganin marasa lafiya, gami da gano ƙwayoyin rigakafi na IgM da ma'aunin rigakafi na IgG.Magungunan rigakafi na IgM suna ɓacewa a cikin makonni da yawa, yayin da ƙwayoyin rigakafi na IgG suka ci gaba har tsawon shekaru.Ƙaddamar da gano kamuwa da ƙwayar cuta ta kwayar cuta yana cika ta hanyar serologically ta hanyar nuna haɓakar titer na antibody zuwa ƙwayar cuta ko kuma ta hanyar nuna ƙwayoyin rigakafin ƙwayoyin cuta na ajin IgM.Hanyoyin da aka yi amfani da su sun haɗa da gwajin tsaka-tsaki (Nt), gwajin ƙaddamarwa (CF), gwajin hanawa na hemagglutination (HI), da gwajin immunofluorescence (IF), m hemagglutination, da immunodiffusion.

A. Ƙididdigar Neutralization

A lokacin kamuwa da cuta ko al'adar tantanin halitta, ƙwayoyin cuta na iya hana su ta takamaiman maganin rigakafi da kuma rasa kamuwa da cuta, irin wannan rigakafin ana bayyana shi azaman antibody neutral.Ƙididdiga na tsaka-tsaki shine don gano antibody neutralization a cikin maganin marasa lafiya.

B. Ƙwararren Ƙwararren Ƙwararru

Za a iya amfani da gwajin gyarawa don nemo gaban takamaiman antibody ko antigen a cikin maganin majiyyaci.Gwajin yana amfani da sel jajayen jinin tumaki (SRBC), anti-SRBC antibody da ƙari, tare da takamaiman antigen (idan neman antibody a cikin jini) ko takamaiman antibody (idan neman antigen a cikin jini).

C. Hemagglutination Inhibition Assays

Idan yawan ƙwayar ƙwayar cuta a cikin samfurin yana da girma, lokacin da aka haɗa samfurin tare da RBCs, za a yi lattice na ƙwayoyin cuta da RBCs.Wannan sabon abu ana kiransa hemagglutination.Idan antibodies a kan hemagglutinins suna nan, za a hana hemagglutination.A lokacin gwajin hana hemagglutination, serial dilutions na serum ana haɗe shi da adadin ƙwayar cuta da aka sani.Bayan shiryawa, ana ƙara RBCs, kuma ana barin cakuda ya zauna na sa'o'i da yawa.Idan an hana hemagglutination, pellet na RBCs yana samuwa a kasan bututu.Idan ba a hana hemaglutination ba, an samar da fim na bakin ciki.