Daga Janairu 2020 zuwa Oktoba 2020, an gudanar da bincike mai yiwuwa a Asibitin Jami'ar Pisa wanda aka buga akan BMC Microbiology.Goldstream®An yi amfani da gwajin Fungus (1-3) -β-D-Glucan don gano matakin BDG daga samfuran BAL.An ƙididdige sakamakon ta aCikakken Mai karanta Kinetic Tube Reader IGL-200daga Era Biology.Binciken ya nuna cewa ya kamata a yi la'akari da BDG don ƙimar ƙimar da ba ta da kyau.Kuma ya haifar da amfani wajen kawar da cutar ta PCP ga duk marasa lafiya marasa lafiya.
Bayani:
Matsayin gwal na bincike na yanzu donPneumocystis jijiyaAna wakilta ta ta hanyar hangen nesa na naman gwari daga samfuran numfashi na asibiti, kamar ruwan bronchoalveolar-lavage, yana bayyana "tabbatar"P. jiroveciiciwon huhu, yayin da qPCR yana ba da damar ma'anar "mai yiwuwa" ganewar asali, saboda ba zai iya nuna bambancin kamuwa da cuta daga mulkin mallaka.Koyaya, hanyoyin kwayoyin, irin su PCR na ƙarshe da qPCR, suna da sauri, sauƙin aiwatarwa da fassara, don haka ba da damar dakin gwaje-gwaje don mayar da bayanan ƙwayoyin ƙwayoyin cuta masu amfani a cikin ɗan gajeren lokaci.Binciken na yanzu yana nufin kwatanta microscopy tare da gwaje-gwajen kwayoyin halitta da aikin bincike na beta-D-glucan akan ruwan bronchoalveolar-lavage daga marasa lafiya da ake zargi.Pneumocystis jijiyanamoniya.Ruwan Bronchoalveolar-lavage daga babban haɗari goma sha takwas da batutuwan sarrafawa mara kyau guda huɗu sun shiga Grocott-Gomori's methenamine silver-tabon, PCR na ƙarshe, RT-PCR, da beta-D-glucan assay
Sakamako:
Duk samfuran da ba su da kyau na bronchoalveolar-lavage (50%) suma sun haifar da tabbatacce ta hanyar ƙarshen lokaci da ainihin lokacin PCR da duka, amma biyu, sun haifar da tabbatacce kuma ta hanyar ƙididdigar beta-D-glucan.PCR-Karshen PCR da RT-PCR sun gano 10 (55%) da 11 (61%) daga cikin samfuran 18, bi da bi, don haka yana nuna haɓakar haɓakar hankali idan aka kwatanta da abin ƙira.Duk RT-PCR tare da Ct<27 an tabbatar da su ta microscopically, yayin da samfurori tare da Ct≥ 27 ba su kasance ba.
Ƙarshe:
Ayyukanmu yana nuna buƙatar sake fasalin da sake fasalin rawar binciken kwayoyin halitta a cikin wani wuri na musamman na asibiti, kamarP. jiroveciikamuwa da cuta, wanda ba kasafai ba ne amma kuma mai tsanani kuma mai saurin ci gaba yanayin asibiti wanda ke shafar rundunonin da ba su da rigakafi wanda zai fi fa'ida daga gano cutar da sauri.Zaɓaɓɓen marasa lafiya, bisa ga ƙa'idodin haɗawa, sakamakon mummunan ta hanyoyin ƙwayoyin cuta za a iya yanke hukunci donP. jiroveciinamoniya.
Franconi I, Leonildi A, Erra G, et al.Kwatanta hanyoyin microbiological daban-daban don gano Pneumocystis jirovecii ciwon huhu akan ruwan bronchoalveolar-lavage.BMC Microbiol.2022;22 (1):143.An buga 2022 Mayu 21. doi:10.1186/s12866-022-02559-1
Lokacin aikawa: Juni-14-2022